Chapter 3

The purpose of Chapter 3 is to produce the recombinant plasmid A with the red fluorescent protein gene (rfp), which can make the red fluorescent protein in bacteria. Students use restriction enzymes to cut two plasmids, which will generate DNA fragments, i.e. restriction digestion. The fragments are then linked by performing the ligation reaction. The lengths of the products from the restriction digest and the ligation are examined by gel electrophoresis.

Lab 3.1: Cutting the Two Plasmids (Restriction Digestion)

Lab 3.2: Putting the rfp Gene into the Plasmid (Ligation)

Lab 3.3: Confirmation by Gel Electrophoresis (Digestion and Ligation Products)